Reddit Reddit reviews CRISPR: Methods and Protocols (Methods in Molecular Biology (1311))

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CRISPR: Methods and Protocols (Methods in Molecular Biology (1311))
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1 Reddit comment about CRISPR: Methods and Protocols (Methods in Molecular Biology (1311)):

u/SmorgasConfigurator ยท 1 pointr/biotech

Short answer is that after some research it is clear we are in a bit of a method explosion, where methods are developed for specific systems, rather than as a readily transferable toolbox. That makes sense, we are yet to discover which type of protocol proves the most efficacious.

A partial collection:

  • For various reasons I am interested in fungi and this article goes into CRISPR for that system.
  • I did find a CRISPR laboratory manual, but I don't have access so I couldn't make any assessment of its utility or detail.
  • Springer also have a book on CRISPR protocols, but again one I can't access to evaluate.
  • Then an early paper that goes really deep into a protocol was found. However, I suspect best practices have evolved since.

    But this is by no means exhaustive and just an observation of what may be available.