Reddit reviews Corning 430049 Polystyrene Bottle Top Vacuum Sterile Filter, Nylon Membrane, 0.2 Micron, 45mm Bottle Neck Diameter, 500mL Capacity (Case of 12)
We found 2 Reddit comments about Corning 430049 Polystyrene Bottle Top Vacuum Sterile Filter, Nylon Membrane, 0.2 Micron, 45mm Bottle Neck Diameter, 500mL Capacity (Case of 12). Here are the top ones, ranked by their Reddit score.
Bottle top vacuum filter is designed to fit plastic or glass media bottles with 45mm neck sizesNylon (NY) membrane is naturally hydrophilic, surfactant-free, and offers the lowest extractables; membrane has a 0.22m pore size and a 33.2sq cm areaPolystyrene filter funnel has a 500mL capacity and a 45mm diameter polyethylene neck adaptor with an angled hose connector that simplifies vacuum line attachmentIndividually packaged, sterile, and certified nonpyrogenic; designed for filtering and storing cell culture media, biological fluids, and other aqueous solutions
To illustrate what BST said...
Bottle Top filter example: http://www.amazon.com/Corning-430049-Polystyrene-Membrane-Diameter/dp/B005J5BVBI
Media Bottle: http://www.amazon.com/PYREX-500mL-Round-Storage-Bottles/dp/B004XR5TUM/ref=sr_1_1?s=industrial&ie=UTF8&qid=1421326439&sr=1-1&keywords=borosilicate+media+bottle.+45mm&pebp=1421326442468&peasin=B004XR5TUM
For autoclaving, in college we would just cover the top of the bottle with a small piece of aluminum foil and secure it with autoclave tape (which will also indicate when a specific temp has been reached)
Tape: http://en.wikipedia.org/wiki/Autoclave_tape
So I figured out why my bottle top filters wouldn't hold the vacuum, and it was simply because it wasn't airtight. I had to push the top against my thigh to get an airtight seal and only then did it hold a vacuum.
So basically, how do I make the top airtight? It only came with these crappy plastic dust covers. I've got these filters specifically, if it helps.
*Also, the filters are plastic, which I didn't factor in when I decided to buy them. Are they still good to use, considering they'll be under pressure? If so, how much pressure can I safely use?