Reddit reviews SEOH 10ml Class B Mohr Pipets
We found 3 Reddit comments about SEOH 10ml Class B Mohr Pipets. Here are the top ones, ranked by their Reddit score.
Capacity (ml) 10Graduation Interval (ml) 0.1Borosilicate Glass Class BType Mohr, To DeliverSold As Each
Homebrew Materials:
Vacuum Pump Oil
Vacuum Tubing
1/4" Barb x 1/4" NPT Male Hose Fitting
1/4" Male x 1/4" Female NPT Extension Hose
Vacuum Pump
Filters
Spatulas
Magnetic Stir Bar
Digital Hotplate with Magnetic Stirrer
Nitrile Gloves (Better than Latex)
Empty Capsules (Buy Separated unless you hate your life)
Capsule Machine (If you value your sanity, buy more than a 100... I hated my life doing this)
Mohr Pipets
Pipette 10ml
Mortar and Pestle (If you're doing orals, get this... Otherwise, you're playing Russian Roulette with your doses and liver)
Funnel (For pouring into small places)
Alconox (For clean your shit)
Temperature Gun (How hot is my shit?)
Graduated Cylinder (If you measure with beakers, you're a fucking idiot... And you should consider Volumetric Flasks)
Beaker Set
1 Liter Beaker (Because brewing en'masse is better)
Scale (I have a 2kg one, too)
Calibration Weight Set
Calibration Weight 100g (Because you need two for the scale linked)
Vacuum Regulator (Unless you want to filter 9001 times due to broken shit)
Oil (Any USP grade, filtered or unfitlered)
BB
BA
Butyl Stoppers
Flip Top Seals
Crimper
Vials (Unsealed)
Autoclave (Cheaper than an oven, why are you using an oven?!)
Weight Boats (Buy a million of these. They're cheap and useful)
Did I miss something? Probably. Yes.
Yeah... flavoring only.
I've tried using it for VG and it was just too fucking annoying.
http://www.amazon.com/Karter-Scientific-Release-Pipette-Pipettor/dp/B005Z4QY2E/ref=sr_1_2?ie=UTF8&qid=1368235487&sr=8-2&keywords=pipette+pump
and
http://www.amazon.com/Mohr-Pipets-Class-B-10ml/dp/B00191LTA4/ref=sr_1_2?ie=UTF8&qid=1368235514&sr=8-2&keywords=10ml+pipette
Are how I pull base. You just need to put a strong rubber band around the quick release because VG is so viscous that pushing the plunger can cause the quick release to open.
My clean environment is simply a glass top table that I wipe down with 10% bleach and 70% ethanol with a little tiki torch burner and I've had great success with minimal contams (2 out of 40 plates). I'm starting LC using a $7 pipettor with a $10 reusable glass serological pipet for transferring between LC and grain jars. I'll also use an inoculating loop to transfer between spore print to agar or agar culture to LC. It's just some methods I'm adopting from things I've learned in microbiology lab courses so I can avoid using disposable syringes.