(Part 2) Best biochemistry books according to redditors

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We found 268 Reddit comments discussing the best biochemistry books. We ranked the 131 resulting products by number of redditors who mentioned them. Here are the products ranked 21-40. You can also go back to the previous section.

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Top Reddit comments about Biochemistry:

u/ninjafizzy · 239 pointsr/funny

All of the books I can see from top to bottom on Amazon:

  1. http://www.amazon.com/Elements-Chemical-Reaction-Engineering-Edition/dp/0130473944 -- used price: $90.98.
  2. http://www.amazon.com/Molecular-Thermodynamics-Donald-McQuarrie/dp/189138905X/ref=sr_1_1?s=books&ie=UTF8&qid=1407531821&sr=1-1&keywords=molecular+thermodynamics -- used price: $70.00 (paperback is $29.99)
  3. http://www.amazon.com/Physical-Chemistry-Molecular-Donald-McQuarrie/dp/0935702997/ref=sr_1_1?s=books&ie=UTF8&qid=1407531925&sr=1-1&keywords=physical+chemistry+a+molecular+approach -- used price: $72.44 (paperback is $42.65)
  4. http://www.amazon.com/Quantum-Physics-Molecules-Solids-Particles/dp/047187373X/ref=sr_1_1?s=books&ie=UTF8&qid=1407532022&sr=1-1&keywords=quantum+physics+of+atoms+molecules+solids+nuclei+and+particles -- used price: $52.66
  5. http://www.amazon.com/Introduction-Chemical-Engineering-Thermodynamics-Mcgraw-Hill/dp/0073104450/ref=sr_1_1?s=books&ie=UTF8&qid=1407532094&sr=1-1&keywords=introduction+to+chemical+engineering+thermodynamics -- used price: $129.96 (paperback is $84.38)
  6. http://www.amazon.com/Organic-Chemistry-8th-Eighth-BYMcMurry/dp/B004TSKJVE/ref=sr_1_5?s=books&ie=UTF8&qid=1407532227&sr=1-5&keywords=organic+chemistry+mcmurry+8th+edition -- used price: $169.33 (paperback is $79.86)
  7. http://www.amazon.com/Elementary-Differential-Equations-William-Boyce/dp/047003940X/ref=sr_1_7?ie=UTF8&qid=1407532549&sr=8-7&keywords=Elementary+Differential+Equations+and+Boundary+Value+Problems%2C+9th+Edition+solutions -- used price: $8.00
  8. http://www.amazon.com/Numerical-Methods-Engineers-Sixth-Edition/dp/0073401064/ref=sr_1_1?ie=UTF8&qid=1407532859&sr=8-1&keywords=numerical+methods+for+engineers+6th+edition -- used price: $47.99 (paperback is $22.48)
  9. http://www.amazon.com/Applied-Partial-Differential-Equations-Mathematics/dp/0486419762/ref=sr_1_5?s=books&ie=UTF8&qid=1407532927&sr=1-5&keywords=applied+partial+differential+equations -- used price: $8.32 (paperback is $1.96)
  10. http://www.amazon.com/Transport-Phenomena-2nd-Byron-Bird/dp/0471410772/ref=sr_1_1?s=books&ie=UTF8&qid=1407533036&sr=1-1&keywords=transport+phenomena+bird+stewart+lightfoot+2nd+edition -- used price: $28.00
  11. http://www.amazon.com/Basic-Engineering-Data-Collection-Analysis/dp/053436957X/ref=sr_1_2?s=books&ie=UTF8&qid=1407533106&sr=1-2&keywords=data+collection+and+analysis -- used price: $80.00
  12. http://www.amazon.com/Calculus-9th-Dale-Varberg/dp/0131429248/ref=sr_1_1?s=books&ie=UTF8&qid=1407533219&sr=1-1&keywords=calculus+varberg+purcell+rigdon+9th+edition+pearson -- used price: $11.97 (paperback is $2.94)
  13. http://www.amazon.com/Elementary-Principles-Chemical-Processes-Integrated/dp/0471720631/ref=sr_1_1?s=books&ie=UTF8&qid=1407533286&sr=1-1&keywords=elementary+principles+of+chemical+processes -- used price: $161.72
  14. http://www.amazon.com/Inorganic-Chemistry-4th-Gary-Miessler/dp/0136128661/ref=sr_1_1?s=books&ie=UTF8&qid=1407533412&sr=1-1&keywords=inorganic+chemistry+messler -- used price: $75.00
  15. http://www.amazon.com/Fundamentals-Heat-Transfer-Theodore-Bergman/dp/0470501979/ref=sr_1_1?s=books&ie=UTF8&qid=1407533484&sr=1-1&keywords=fundamental+of+heat+and+mass+transfer -- used price: $154.99 (loose leaf is $118.23)
  16. http://www.amazon.com/Biochemistry-Course-John-L-Tymoczko/dp/1429283602/ref=sr_1_1?s=books&ie=UTF8&qid=1407533588&sr=1-1&keywords=biochemistry+a+short+course -- used price: $139.00 (loose leaf is $115)
  17. http://www.amazon.com/Separation-Process-Principles-Biochemical-Operations/dp/0470481838 -- used price: $93.50 (international edition is $49.80)
  18. http://www.amazon.com/University-Physics-Modern-13th/dp/0321696867/ref=sr_1_1?s=books&ie=UTF8&qid=1407545099&sr=1-1&keywords=university+physics+young+and+freedman -- used price: $83.00

    Books & Speakers | Price (New)
    ---|---
    Elements of Chemical Reaction Engineering (4th Edition) | $122.84
    Molecular Thermodynamics | $80.17
    Physical Chemistry: A Molecular Approach | $89.59
    Quantum Physics of Atoms, Molecules, Solids, Nuclei, and Particles | $128.32
    Introduction to Chemical Engineering Thermodynamics (The Mcgraw-Hill Chemical Engineering Series) | $226.58
    Organic Chemistry 8th Edition | $186.00
    Elementary Differential Equations | $217.67
    Numerical Methods for Engineers, Sixth Edition | $200.67
    Applied Partial Differential Equations | $20.46
    Transport Phenomena, 2nd Edition | $85.00
    Basic Engineering Data Collection and Analysis | $239.49
    Calculus (9th Edition) | $146.36
    Elementary Principles of Chemical Processes, 3rd Edition | $206.11
    Inorganic Chemistry (4th Edition) | $100.00
    Fundamentals of Heat and Mass Transfer | $197.11
    Biochemistry: A Short Course, 2nd Edition | $161.45
    Separation Process Principles: Chemical and Biochemical Operations | $156.71
    University Physics with Modern Physics (13th Edition) | $217.58
    Speakers | $50.00

    Most you can get is $1476.86 (selling all of the books (used and hard cover) in person), and if you sell it on Amazon, they take around 15% in fees, so you'll still get $1255.33. But wait...if you sell it to your university's book store, best they can do is $.01.

    Total cost: $2832.11 (including speakers)

    Net loss: -$1355.25 (books only). If sold on Amazon, net loss: -$1576.78 (books only). Speakers look nice; I wouldn't sell them.

    Edit: Added the two books and the table. /u/The_King_of_Pants gave the price of speakers. ¡Muchas gracias para el oro! Reminder: Never buy your books at the bookstore.

    Edit 2: Here are most of the books on Library Genesis
    Thanks to /u/WhereToGoTomorrow
u/seattlebiotech · 24 pointsr/biology

You gotta go check out the Protein Data Bank. It's pretty much *the* source for information on proteins. They have a "protein of the month" that they write about and explain all sorts of cool things about. <https://www.rcsb.org/\>

If you can get your hands on a copy buy "The machinery of Life" <https://www.amazon.com/Machinery-Life-David-S-Goodsell/dp/0387982736\> a book that's a really clear but accurate and detailed explanation of how proteins are made in cells, how DNA and proteins are related, and a lot of other concepts explained very clearly. It's like all the cool parts of what you learn in a college level biochemistry course, but way easier to understand and with less tests and math!

Also check out the game "Fold It" <https://fold.it/portal/\>. It's a game where you fold and design proteins. If you like that try to install a protein design software like Rosetta <https://www.rosettacommons.org/\>.

Lots of cities have shared bio-hacker-spaces where you can use their equipment and meet other people. When I was your age I got involved in robotics by getting involved with a local community group. I learned a lot and people helped me out and By the time I graduated from high-school I had designed robot parts that were sold around the world by the top robot part manufacturer in the US. You can do anything when you are your age and you are interested in something.

The number one thing I recommend is to keep being curious, spend every free moment you can exploring, and when you cant figure out how to do something (because you don't know how, don't have the money, or don't have the equipment) don't let that stop you. Just politely ask people for help and you might not always get a reply but often you will.

Read a lot, learn a lot, watch documentaries, and when you have an idea for a protein you could make email some college professors at whatever colleges are around you and ask if they can help you accomplish your goal. It's pretty likely they will help you.

​

PS: Here are some great places to start:

Gene design competition for students: http://2008.igem.org/Main_Page

Universal genes (instructions for making proteins) https://biobricks.org/freegenes/

(Pipetting robot) https://opentrons.com

(The best Protein/Biochem lectures) https://www.youtube.com/watch?v=ZfsuDfui0oU

(Protein design program) https://els.comotion.uw.edu/express_license_technologies/rosetta

(Home made biology tools) https://pipettejockey.com/

(VR biology viewer) https://store.steampowered.com/app/493430/Nanome/

u/Flying_Cat · 11 pointsr/Biochemistry

Here are some resources/tips that I've found helpful in my time working in a crystallography lab.

  • Crystallography made Crystal Clear - good overview https://www.amazon.com/Crystallography-Made-Crystal-Clear-Macromolecular/dp/0125870736/ref=sr_1_1?ie=UTF8&qid=1536938710&sr=8-1&keywords=Crystallography+made+crystal+clear
  • Tererese Bergfors website: http://xray.bmc.uu.se/terese/tutorials.html Good tutorials
  • Look for Studier 2002- his formulations for Auto-induction media. Of interest is the formulation for PASM-5052 should you need to do experimental phasing via Selenomet labeling. As a side note, always look up the percent sequence identity of your protein to its homologs in the PDB. If its below 40%, definitely plan to do experimental phasing. Even at that, some labs have started hopping straight to expressing and crystallizing Selenomethionine labeled protein and collecting on the selenium edge from the get-go so that they hedge their bets on phasing. If doing experimental phasing, always collect until your crystal is dead dead dead.
  • Most common issue with crystallization in my experience is a sample prep issue. This paper was very helpful to me when troubleshooting protein prep quality issues. http://dx.doi.org/10.1016/j.febslet.2013.10.044 One of my proteins was aggregating on the hours-days timescale. Don't neglect the utility of SEC, even if you see a single band on an SDS gel, you may have a mixture of oligomeric states which can only be detected by looking at the chromatogram from an SEC run.
  • Look at the drops that do crystallize, then look at the neighbors in the same tray that don't have crystals. These observations can make it easier to figure out when you're close to the right crystallization conditions.
  • High purity reagents- It's 2 O'clock in the morning, what is the purity of your Tween 20? 40%....40 fucking %. This is from Sigma aldrich, not some po dunk little chemical firm. Document your manufacturer if you need to use a detergent or reagent with purity this low. Regardless, document your fucking manufacturers of your reagents. One of the grad students in my lab was using TCEP and even though the purity was fairly high, a change of manufacturer stopped his protein from crystallizing. We also had a similar incident with a syringe filter manufacturer change.
  • Submit samples for High-throughput screening at multiple concentrations, also recommended is to document the buffer your protein is in and try multiple different buffers so long as you get a clean chromatogram from SEC. Sometimes one buffer system works better than another for getting good quality crystals.
  • Generally if possible, avoid glycerol. If your protein is having aggregation issues due to hydrophobic interactions, use a high-purity detergent like CHAPS, or n-Octyl-β-D-Glucopyranoside, or even sometimes an organic solvent like DMSO or isopropanol. All of the above mentioned compounds are the most cost-effective in my experience.
  • Don't neglect the role of ligands. Sometimes you need to add a ligand or product to lock down the conformation.
  • Don't neglect activity assays to make sure your sample preps are producing active protein.
  • Don't count on an NMR structure to phase a crystal structure. I made this mistake, same protein, identical sequence, unable to phase with molecular replacement. I ultimately had to do a SAD experiment to phase.
  • If your protein is badly behaved, you can try to rescue it with a maltose binding protein tag or a SUMO tag, etc. Sometimes they're helpful.
  • DNA-binding proteins are often unstable in low salt when not bound to their target DNA sequence. Try binding the protein to its target DNA sequence then buffer exchanging into a low salt buffer.
  • Seeding can sometimes be helpful.
  • Make sure your sample purity is very high, the higher the better. Heterogeneity will fuck you on diffraction.
  • Don't judge a book by its cover. Some crystals are ugly and diffract beautifully, others look beautiful and diffract terribly. I had these beautiful hexagonal crystals- no diffraction. In contrast, I had these plates that I expected nothing of that diffracted out to 1.7Angstroms.
  • Common issue that can stop you from phasing is processing in the incorrect space group.
  • Don't bother with HKL-2000 or HKL-3000. I strongly prefer XDS for data processing as it doesn't fail silently. There was a crystal dataset I was working on that had strong space group ambiguity and HKL-2k just wouldn't process it. XDS gave me enough feedback I was able to correctly diagnose and correct the issue. XDS is command line based but it gives you a lot more control and feedback.
  • Get cozy with Phenix and Coot.

    I hope this helps. Let me know if you have any questions.
u/mischief_____managed · 9 pointsr/medlabprofessionals

I just passed it last month on my first try. What really helped me was this Lela Buckingham book as well as this Indeed forum. My best advice to you is to go through the entire forum, all 1300+ post from the very beginning and compile a list of questions and topics that people mention and study that rigorously. 60% of my exam consisted of questions I had already seen on the forum so I felt really confident! I focused mainly on that and also read up on topics in the book that I was not too familiar with!

u/Chemweeb · 7 pointsr/chemistry

Try to look for information about telomerase, DNA polymerase and how DNA replication occurs in the body. You should find your answers there.

If you want to have a good book, 'From Genes to Genomes' should give you a good introduction into biochemistry, including these themes.

Here's an amazon link to the 5th edition, but there's of course other options:
http://www.amazon.com/Genetics-Genomes-Leland-H-Hartwell/dp/0073525316

u/livebythem · 7 pointsr/molecularbiology

Molecular Biology of the cell - Great textbook to get you started. It is really comprehensive but not challenging to read. The diagrams are informative but not overbearing. The author clearly cares a great deal about the subject.

https://www.amazon.com/Molecular-Biology-Cell-Bruce-Alberts/dp/0815341059

Molecular Biology - Weaver - This one is nice because it keys in on many of the landmark experiments and scientists who contributed greatly to the field:

https://www.amazon.com/Molecular-Biology-Associate-College-Sciences/dp/0073525324/ref=sr_1_1?crid=1L89I1QHNC7HX&keywords=molecular+biology+weaver&qid=1571969517&sprefix=molecular+biology+weaver%2Caps%2C130&sr=8-1

If you want something smaller and more like a narrative, give Recombinant DNA: Genes and Genomes - A Short Course a try.

https://www.amazon.com/Recombinant-DNA-Genomes-Course-Edition/dp/0716728664

u/JuniorOneNut · 6 pointsr/Biochemistry

I took a chemical and synthetic biology class last semester, we used a textbook called The Organic Chemistry of Biological Pathways. It was pretty good but I feel like it relied on you having a pretty good organic background.

u/Whisky4Breakfast · 6 pointsr/AdvancedFitness

The first overall source I'd look to for Ex-Sci is a textbook from Mcardle Katch & Katch it's a bit more user friendly for getting into the field.

Another good source for info is the American College of Sports Medicine (ACSM), and they have an Intro to Exercise Science as well. They're a bit more Science and Research Heavy, so they can be good or bad depending on the reader.

To get a good starter for musculature a very helpful one is Strength Training Anatomy This one is only a very colorful and visual source of where the different muscles are and how they're involved with different movements.

Supertraining was mentioned earlier in the thread, and is an Amazing source for how different training variables and methods affect the body.

I've found Exercise Metabolism very helpful in how the body uses different macro-nutrients in various intensities of physical activity.

One of my favorite books is also the Essentials of Strength and Conditioning from the National Strength and Conditioning Association (NSCA). It's more geared toward programming for athletic pursuits rather than overall physical fitness, but it still does give a great understanding of training variables and the body's adaptations to them.

EDIT: The subject of Kinesiology is touched on in most resources, but you may also want to get a standalone resource for this if you want to really understand the construction and functionality of the musculoskeletal system. The courses I've taken and research I've done have used a lot of different resources, so I don't have a single one personally to include here.

u/Yoschwa · 4 pointsr/genetics

I'm a big fan of Hartwell et al, Genetics: From Genes to Genomes. In particular, the problems at the end of each chapter are the best I saw in any intro textbook.

u/Anabaena_azollae · 3 pointsr/Biochemistry

I have pretty limited experience with more advanced enzyme kinetics, but the scenario you described seems pretty straightforward and I imagine it would be covered in pretty much any book on the subject. I've used Cornish-Bowden's Fundamentals of Enzyme Kinetics as a reference before and found it to be well written and clear. I know Segel is kind of an encyclopedia of enzyme kinetics and is certain to have the formulas you need, though might not be best as a text to actually learn the subject properly.

u/Furthur · 3 pointsr/triathlon

great place to start

It's pretty neat what you get out of half a semester of graduate level exercise physiology

u/danesgod · 3 pointsr/chemistry

Here are my top three textbook choices:

This is a great book for how to actually do organometallic syntheses. I've followed several procedures in the book, and they work well / are quite descriptive.

I''ve taken two organometallic courses that use Crabtree. So I think this is a great book to learn organometallics. Definitely more organometallic than Miessler and Tarr.

A few of my friends have the new Hartwig book which looks awesome. I haven't read it yet, but in my opinion its going to surpass Crabtree as the textbook of choice for organometallics classes in the next few years.

u/smoothtrip · 2 pointsr/askscience

Indeed we can. http://en.wikipedia.org/wiki/Population_inversion

In particular you might be interested in http://en.wikipedia.org/wiki/Population_inversion#Creating_a_population_inversion.

and

http://en.wikipedia.org/wiki/Time-resolved_spectroscopy#Transient-absorption_spectroscopy

Other resources that are extremely helpful are:

http://www.amazon.com/Principles-Fluorescence-Spectroscopy-Joseph-Lakowicz/dp/0387312781

If you really want to get into more advance topics in lasers, a book on non-linear optics is very helpful. I am drawing a blank right now on a good book. I will try to post a book tomorrow in the morning if I remember.

Edit: Looks like someone else posted a link for a Non-linear optics book.

u/sab3r · 2 pointsr/worldnews

There's still a lot of research on mechanosensation in plants (it's not even well understood in animals yet) but there's reasonable evidence to say that plants can indeed feel a form of pain, so to speak. Here's a simple video on the subject. There's also an entire book on this subject: Communication in Plants: Neuronal Aspects of Plant Life.

Some interesting paragraphs from the introduction:

>…Each root apex is proposed to harbor brain-like units of the nervous system of plants. The number of root apices in the plant body is high, and all ‘brain units’ are interconnected via vascular strands (plant neurons) with their polarly-transported auxin (plant neurotransmitter), to form a serial (parallel) neuronal system of plants. From observation of the plant body of maize, it is obvious that the number of root apices is extremely high, whereas there are only few shoot apices. This feature makes the ‘serial plant brain’ extremely robust and the amount of processed information must be immense.

>Our view of plants is changing dramatically, tending away from seeing them as passive entities subject to environmental forces and organisms that are designed solely for accumulation of photosynthetic products. The new view, by contrast, is that plants are dynamic and highly sensitive organisms, actively and competitively foraging for limited resources both above and below ground, and that they are also organisms which accurately compute their circumstances, use sophisticated cost-benefit analysis, and that take defined actions to mitigate and control diverse environmental insults. Moreover, plants are also capable of a refined recognition of self and non-self and this leads to territorial behavior. This new view considers plants as information-processing organisms with complex communication throughout the individual plant. Plants are as sophisticated in behavior as animals but their potential has been masked because it operates on time scales many orders of magnitude longer than that operating in animals.

u/mehblah666 · 2 pointsr/Biochemistry

Crystallography Made Crystal Clear is a fantastic resource for learning the theory behind protein crystallography and structure solution. I used it to learn when I was starting out and I still consult it at times.

Crystallography Made Crystal Clear: A Guide for Users of Macromolecular Models (Complementary Science) https://www.amazon.com/dp/0125870736/ref=cm_sw_r_cp_api_Rp9MBb4PTEANT

Good luck!


Beyond this, I’ll add that there’s really no substitute for doing this hands on and learning from other students/postdocs in the lab. Crystallization feels like black magic a lot of the time, so the theory only gets you so far. Most of the learning really happens as you go along.

u/gudgeonpin · 2 pointsr/chemistry

For bioinorganic, I've used a couple of texts in my classes.

Any book that Harry Gray works on is good in my eyes (I'm biased):
This is <a href="http://www.amazon.com/Biological-Inorganic-Chemistry-Structure-Reactivity/dp/1891389432/ref=sr_1_1?s=books&ie=UTF8&qid=1376182276&sr=1-1&keywords=bioinorganic+chemistry">one example</a>

But Lippard and Berg also have a very good treatise on bioinorganic chemistry: Another <a href="http://www.amazon.com/Principles-Bioinorganic-Chemistry-Stephen-Lippard/dp/0935702725/ref=sr_1_2?s=books&ie=UTF8&qid=1376182276&sr=1-2&keywords=bioinorganic+chemistry">Amazon link</a>


sorry- I'm obviously weak on the html. Tried to follow the formatting help, but I'm missing something.

u/speckledlemon · 2 pointsr/chemistry

Maybe try this?

u/nuchemist · 2 pointsr/chemistry

If that is the chemistry you're interested in, this is a good book to get you started. Buy it and read it.

u/praxeologue · 2 pointsr/medlabprofessionals

No problem. Since that list is pretty long, I could even narrow it down a bit.

Microbiology

Transfusion Medicine

Clinical Chemistry

Histotechnology

Hematology

Urinalysis

Molecular Diagnostics

Specimen Procurement

Some of these you can even find free PDFs of online, if you're savvy.

u/useless83 · 2 pointsr/chemhelp

This is the book I used and I really liked it. Has lots of trends and explains things in easy to follow terms.

http://www.amazon.com/Organometallic-Chemistry-Transition-Metals/dp/0470257628

u/phindingphilemon · 2 pointsr/primerlearning

Evolutionary genomics/genetics, population/quantitative genetics. A quick google search will get you started. Fair amount of math, mostly statistics, a bit of Taylor rule stuff. The classics are still highly regarded (Fisher, Haldane, Wright, Falconer). [Here] (https://www.gnxp.com/WordPress/2017/06/27/why-you-should-learn-some-population-genetics/) is a good starter pack compiled by someone who knows what he's talking about.

Re: 'survival of the stable' Addy Pross put forth a theory of biogenesis that roughly parallel's the idea in his [What is Life? How Chemistry Becomes Biology] (https://www.amazon.com/What-Life-Chemistry-Becomes-Biology/dp/1522693009). Basically it's a theory of chemical kinetics as a precursor to biological replication. It's a slightly paradoxical take on stability, what he calls KDS (kinetic dynamic stability). The higher a reagent's rate of reactivity, the more products it results in. You can probably guess how he makes the connections to evolution. Not super convincing imo but an interesting read. Much better is Nick Lane's newest book,[The Vital Question] (https://www.amazon.com/Vital-Question-Evolution-Origins-Complex/dp/0393352978/ref=sr_1_1?s=books&ie=UTF8&qid=1527312251&sr=1-1&keywords=nick+lane&dpID=412x3ysIEeL&preST=_SY291_BO1,204,203,200_QL40_&dpSrc=srch)--no math involved but makes for rough sledding unless you know at least some orgo/biochem.

Care to preview a bit of the roadmap we'll be taking in the series helpsypooo?

u/AgentKittenMittens · 2 pointsr/UniversityofReddit

There are a few books, such as The Organic Chemistry of Drug Design and Drug Action by Silverman (a medicinal chemistry textbook) and Classics in Total Synthesis by Nikolaou and Sorenson that would be very specific things. Silverman is very what are inhibitors, what does LD50/ED50 actually mean, SAR analysis, combi-chem, etc. Nikolaou and Sorsenson cover the classic synthesis of things such as erythronolide B, progesterone, strychnine and cocaine (the inactive enantiomer, of course).

There are obviously other resources, but I do really appreciate these texts as they are a part of my personal library.

Source: I have a BS in medicinal chemistry and am a PhD candidate in chemistry (focus in bio-organic for drug design)

u/lju1977 · 1 pointr/Christianity

I studied molecular cell biology for 3 years while at university. I used the standard B.Sc course materials recommended by the professors. Not limited to texts such as Molecular bioology of the cell and Biochemistry

u/[deleted] · 1 pointr/genetics

If you are looking for a very broad overview of the molecular side of genetics, then I second previous comments that suggest Wikipedia. Start here and follow every link. If something isn't terribly clear to you, or you want more information about it, then read some of the journal articles linked in the references. Many of them are avaliable freely online, especially if you are accessing them from a college campus.

For online lecutre notes, you might find MIT's open courseware project to be helpful.

If you are looking specifically for a book to use, then Alberts et al. Molecular Biology of the Cell is considered a 'must have' bible for many practicing biological researchers. I find it a little too low level for deep knowledge. As a deeper alternative to Alberts, I suggest Weaver's Molecular Biology, which covers detailed molecular mechanisms of genetics at several different control levels, as well as including detailed descriptions of original experiments that uncovered the mechanisms, using figures from the original papers.

u/ejm · 1 pointr/bioinformatics

I just started a bioinformatics course at JHU and this is the textbook: https://www.amazon.com/Bioinformatics-Functional-Genomics-Jonathan-Pevsner-ebook/dp/B0144NZ2EC

u/Cpt_Hackum · 1 pointr/chemistry

I'm sure someone with a quantum background could offer a better (and more accurate) explanation.

But as I understand it, the fluorescence lifetime (basically the time between when the fluorescent molecule absorbs a photon and emits a photon) takes a certain amount of time (around 1 to 10 nanoseconds for the dyes we like to label biomolecules with).

While that is a pretty short span of time, the movement of solvent molecules and motions of your protein are quicker. So between when your fluorescent molecule absorbs a photon and emits a photon, the surrounding atoms end up re-orienting and changing the local environment which effects the energy difference between the excited and ground states of the fluorescent molecule.

This change in energy levels follow a Gaussian looking distribution which results in a Gaussian looking spectra. So that's why instead of a a single wavelength emission profile you get a broad spectrum.

Now if your puzzled by the fact that the maxima of the different peaks in the graph you posted shift a little, that can be explained by other changes in the local environment, like the change in pH or the fact its attached to different proteins.

Bear in mind that all of this is off the top of my head, and I could be in the worng. If you're really curious I highly recommend you pick up a copy of "the fluorescence bible" it should sort you out in short order.

u/Frikster · 1 pointr/UBC

There isn't a single sentence in your post I don't agree with (^_^).

Well, except for one:

As a cognitive systems alumni I'd say you'd need to operationalize what "sentient" means before you say plants are not sentient. There is quite a bit of literature on plant intelligence. Plant Neurobiology and plant cognition are both actual fields. Plants have analogous processes to what you might see in a rudimentary brain and hell, some even use neurotransmitters to communicate and problem solve. The link is for hormones but acetylcholine esterase, glutamate receptors, GABA receptors, and endocannabinoids are all used by plants as outlined in this 2006 book

I used to be pescatarian because I cared about mammals but found it incredibly difficult to feel anything for fish - creatures so drastically different from us. Also where was the evidence that they feel pain in any capacity we should care about? That's where research I read made me change my mind. Turns out there's good evidence most fish feel pain and most are more intelligent than I gave them credit for.

I bring this up because I find a lot of vegans categorically think of plants as fundamentally different sentient-wise for no other reason than because they are plants. This seems to me the definition of speciesism. Thinking of sentience as a spectrum with no clear boundary for what is sentient and what isn't seems a healthier worldview more in line with reality. Consider a meat-eater that took enormous great care to cause no pain to the meat he is eating. A preachy vegan walks in and tells him what he is doing is wrong. Consider the meat-eater sharing this quote from the New York Times:

"When a plant is wounded, its body immediately kicks into protection mode. It releases a bouquet of volatile chemicals, which in some cases have been shown to induce neighboring plants to pre-emptively step up their own chemical defenses and in other cases to lure in predators of the beasts that may be causing the damage to the plants. Inside the plant, repair systems are engaged and defenses are mounted, the molecular details of which scientists are still working out, but which involve signaling molecules coursing through the body to rally the cellular troops, even the enlisting of the genome itself, which begins churning out defense-related proteins ... If you think about it, though, why would we expect any organism to lie down and die for our dinner? Organisms have evolved to do everything in their power to avoid being extinguished. How long would any lineage be likely to last if its members effectively didn't care if you killed them?"

Although only suffering animals feel anguish, plants, like all organisms, have evolved mechanisms for survival. No living organism can be described as "wanting" to die for another organism's sustenance. So, if you kill an organism for food without causing it anguish, what's the moral difference between doing that to an animal vs a plant? I don't see one. And doesn't the care I have taken to not cause my meat any pain showcase how much care and respect I have for my food? What respect do you show plants as fellow living organisms by categorically refusing to eat anything but them?

Legitimate questions worth pondering I think. Now, I don't think there are any plants that deserve the label "sentient" and the pain research seems mostly that plants "probably don't feel pain in any way we need to care about" so we're in the clear as vegans/bivalvarians if that's what we care about. But then it follows that there's no moral problem with eating meat if you cause the animal no harm. You cant say "the animal doesn't want to die and has some right to life because of this." The same can be said of plants. Arguing that the animal is just much more "complex" and thus deserves life likewise doesn't work. That is subjective and moreover smells like the same kind of rationale that leads to the irreducible complexity fallacy. Just because something is more complex doesn't magically endow it with mystical properties. It's all molecules and electrical circuits at the bottom for both plants and animals at the end of the day.

However, since ethical meat is virtually impossible to get hold off in the 21st Century, (even the rare ethical farms have serious legal issues that prevent the animals from receiving adequate care) unless I were to go and kill my meat myself, I'll be vegan.

And yea... on a complete sidenote... honey is a topic I'm currently looking into and not completely sure of. Currently I try to only buy local but I don't go out of my way to avoid it. It's probably the most hazy ethical thing in my diet right now and I think it'll probably be the next thing to be removed from my diet if there is a next thing to remove :S . Interesting side-sidenote: Goat is the most eaten meat in the world. And yet... there is no such thing as a goat factory farm (or they're incredibly rare). Lamb likewise is very difficult to factory farm and is not economically feasible so sheep just tend not to be factory farmed (source). not only that but if well managed goat and lamb are easily some of the most environmentally friendly forms of meat. So goat and lamb chops might be returning to my diet one day if I can find an ethical source I can trust!

u/awesome_hats · 1 pointr/genomics

For which one? CRISPR? Well you won't be able to understand the details until you have a good grasp of molecular biology. But molecular biology itself is a huge area. I would like to give you recommendations but it's really hard with your interests so broad.

It is like asking for book recommendations on Linear Algebra: there are tons and some focus on numerical methods, others on eigenvalues, others on decomposition methods like SVD, others on applications in machine learning, etc.

Maybe start with something like this:

https://www.amazon.ca/gp/product/0815344325/ref=pd_lpo_sbs_dp_ss_1/187-9306447-5023949?pf_rd_m=A3DWYIK6Y9EEQB&pf_rd_s=lpo-top-stripe&pf_rd_r=0A3FAK9C4CRAFZQTEQ4H&pf_rd_t=201&pf_rd_p=1977604502&pf_rd_i=0815341059

or this

https://www.amazon.com/Molecular-Biology-Robert-Weaver/dp/0073525324/ref=sr_1_2?ie=UTF8&qid=1479421339&sr=8-2&keywords=Molecular+Biology%3A+Robert+Weaver

for molecular biology.

For introductory genetics, pretty much any undergraduate level biology or biochemistry textbook will help you understand the basics of transcription, translation, genes, heredity, etc. Once you have a thorough understanding of what a gene is, what a regulatory region is, what epigenetics is, what types of mutations occur etc. then you'll have a good idea of which specific questions to ask to explore a certain topic in more detail.

I would also spend time on the wikipedia pages and other sources reading about the different sequencing methods that are central to genetics. Look up Sanger sequencing and explore some of the next-generation-sequencing (NGS) techniques now available. Fully understanding these will also require knowledge of biochemistry, such as phosphodiester bonds, and basic biology techniques and physics such as electrophoresis and fluorescence.

Understanding and diving into CRISPR, genetic engineering, synthetic biology, etc. will require a thorough understanding of the above, and then more detailed knowledge on gene expression, how it is manipulated, about things like plasmids, retroviruses and their molecular machinery, etc.

Then there are people who develop algorithms for bioinformatics that probably know little to none of the above and don't need to for their day to day work; the answer is in general "it depends".

u/locoghoul · 1 pointr/canada

Did you take biochem too? Nevertheless if you did good you should enjoy this one: https://www.amazon.ca/Organic-Chemistry-Biological-Pathways-Second/dp/193622156X

It is good because if you understand how we metabolize anything we eat then you'd have no issue identifying bs from certain claims. Like "fructose is better than sucrose" or "fatty acids are unhealthy" or "you should avoid carbs altogether". It also helps understanding diabetes or other similar pathologies, but I'm sure you kno that already.

u/testuser514 · 1 pointr/engineering

So the answer to your question is buy this book and work through it cover to cover. https://www.amazon.com/BioBuilder-Synthetic-Biology-Natalie-Kuldell/dp/1491904291

This has everything you'll need to understand how what/how/why without needing a PhD. Theres also chapters on ethics and safety when you do the sample syn bio projects given in the book. If you can do the projects given in the book you'll be able to work on larger projects soon.

u/mind_bomber · 1 pointr/Futurology

The book is an easy to read thorough introduction into the field of synthetic biology. It is perfect for enthusiast wanting to know more on the subject or for first time students that want to build a foundation of understanding.

You can read the first 10 pages for free in the amazon link i posted earlier by clicking the image of the book ---> https://www.amazon.com/BioBuilder-Synthetic-Biology-Natalie-Kuldell/dp/1491904291

u/Hryggja · 1 pointr/explainlikeimfive

This isn’t a personal attack, but debating this with people who haven’t read a single nutrition textbook is impossible. I’m talking about the only CICO that anyone could possibly mean in this discussion.

Obviously the human body as a system does not violate the conservation of energy. Not a single person who is sufficiently educated to cite a law of thermodynamics is going to argue that a meal plan can violate it. So I don’t know why you would crusade against a misinterpretation so outlandish that nobody is ever going to present it.

> there is a lot of variety to choose from to find what works for each person.

There’s a functionally infinite number of diets to choose from, in terms of simply which combinations of foods you’re eating, but they’re all just different ways to achieve adequate amounts of a finite number of nutrients necessary for life processes. If you’re getting same nutrient load from one combination of foods or another one, and they’re calorically similar, those diets aren’t really different in the parameters of this topic.

CICO is a reductionist attitude towards nutrition that states that guessing your caloric deficit and staying under it is a viable solution to maintaining a healthy wait over long periods. That claim has been disproven, for decades, I don’t think I’ve ever seen any reputable figure in the field defend it. Playing the CICO game tapers down your metabolic efficiency, and you end up reducing your average burn rate, and having to restrict your caloric intake more and more to avoid a backlash of weight gain, because you’re perpetually keeping your body in a highly anabolic state, and decreasing your insulin sensitivity, so it’s chomping at the bit to store every calorie as fat. The explosion of popular support behind cyclical calorie restriction, which is called intermittent fasting now (16/8 and other feeding windows, “one meal a day”, etc) is still traversing social media, but it’s been a widespread position in nutritional science for, again decades. I can think off-hand of a couple of studies that came out about 20 years ago on Ramadan fasting, which showed transient improvements across several endocrine parameters. The references in this paper are a good resource on the subject.

https://watermark.silverchair.com/znu00407000981.pdf

If you are interested in the subject, here are two texts commonly used in metabolic science courses:

https://www.amazon.com/Advanced-Nutrition-Metabolism-Sareen-Gropper/dp/1305627857

https://www.amazon.com/Biochemistry-Jeremy-M-Berg/dp/1464126100

u/kallassassin7 · 1 pointr/askscience

This book will provide you all of the information you should know as an undergrad, Biochemistry: A Short Course, 2nd Edition:

https://www.amazon.com/Biochemistry-Course-John-L-Tymoczko/dp/1429283602


Focus on learning practical skills on top of book learning. Start from the basics: how to extract DNA (ethanol precipitation), move on to gel electrophoresis then PCR. Understand the protocols, how they can be modified, and why each step is important. Move on to ELISA protocols.

PM me for more info.

u/dhumidifier · 1 pointr/ThingsCutInHalfPorn

From the Machinery Of Life by David S Goodsell. Great book easy for any audience!

http://www.amazon.com/The-Machinery-Life-David-Goodsell/dp/0387982736

u/Com-Boh · 1 pointr/worldnews

Funny enough, Nick Lane just released a book, The Vital Question, on the subject of the possibility of life beginning and evolving in these hydrothermal vents.

u/jeneconnaispas · 1 pointr/NMRspectroscopy

This is the same type of approach I was taught (and is, in my opinion, best explained in Organic Structure Analysis by Crews, but it's also a very expensive book). Here is a pretty good free version for structure determination.


I would also add that the formula C7H11N2 would give an unsaturation number of 3.5, so is not a plausible formula.I'm not trying to be discouraging, but I would highly recommend OP spending some time trying to understand this information. I've graded a lot of lab reports, quizzes and exams and this looks to me like someone who has a little bit of understanding on the material, and is just worried about getting the "right" answer quickly, rather than spending sufficient time understanding why that is the right answer. In my experience, most students need to spend 2-3 times more time studying organic compared to their other classes to really understand the material.

u/wave_mechanic · 1 pointr/chemistry

Here is a link to the Amazon page for it. The reviews seem to be pretty unfavorable (it's never a good sign when the review distribution is centered at 2 stars).

http://www.amazon.com/Organic-Structure-Analysis-Topics-Chemistry/dp/0195336046

Apologies if you've already looked at this.