Best lab diluter dispnsers according to redditors
We found 7 Reddit comments discussing the best lab diluter dispnsers. We ranked the 2 resulting products by number of redditors who mentioned them. Here are the top 20.
We found 7 Reddit comments discussing the best lab diluter dispnsers. We ranked the 2 resulting products by number of redditors who mentioned them. Here are the top 20.
Homebrew Materials:
Vacuum Pump Oil
Vacuum Tubing
1/4" Barb x 1/4" NPT Male Hose Fitting
1/4" Male x 1/4" Female NPT Extension Hose
Vacuum Pump
Filters
Spatulas
Magnetic Stir Bar
Digital Hotplate with Magnetic Stirrer
Nitrile Gloves (Better than Latex)
Empty Capsules (Buy Separated unless you hate your life)
Capsule Machine (If you value your sanity, buy more than a 100... I hated my life doing this)
Mohr Pipets
Pipette 10ml
Mortar and Pestle (If you're doing orals, get this... Otherwise, you're playing Russian Roulette with your doses and liver)
Funnel (For pouring into small places)
Alconox (For clean your shit)
Temperature Gun (How hot is my shit?)
Graduated Cylinder (If you measure with beakers, you're a fucking idiot... And you should consider Volumetric Flasks)
Beaker Set
1 Liter Beaker (Because brewing en'masse is better)
Scale (I have a 2kg one, too)
Calibration Weight Set
Calibration Weight 100g (Because you need two for the scale linked)
Vacuum Regulator (Unless you want to filter 9001 times due to broken shit)
Oil (Any USP grade, filtered or unfitlered)
BB
BA
Butyl Stoppers
Flip Top Seals
Crimper
Vials (Unsealed)
Autoclave (Cheaper than an oven, why are you using an oven?!)
Weight Boats (Buy a million of these. They're cheap and useful)
Did I miss something? Probably. Yes.
I concur with your first week's findings. :-)
As for syringes... you might want to look into the SEOH Pipette pumps along with various sizes of pipettes for them. I absolutely love'em. So much easier to work with and also for the 1ml pipettes very smooth and accurate. Less hassle than syringes.
Also, I had no problem with the viscosity of VG with any size of the pipettes and the pump. Except that I had to push the top instead of using the pump release because VG just doesn't move as fast with just gravity doing its work.
A few links to check out...
http://www.amazon.com/gp/product/B00191BW4C/ref=oh_details_o02_s00_i03?ie=UTF8&psc=1
http://www.amazon.com/gp/product/B00191BXTG/ref=oh_details_o02_s00_i04?ie=UTF8&psc=1
http://www.amazon.com/gp/product/B008J4BR1U/ref=oh_details_o02_s00_i00?ie=UTF8&psc=1
etc. They have various sizes pipettes for these things and also a pump size larger than the 2ml and 10ml ones.
http://www.amazon.com/gp/aw/d/B00191HNKY?pc_redir=1408509882&robot_redir=1
I got this exact one:
http://www.amazon.com/gp/aw/d/B00191BXTG/ref=pd_aw_sims_2?pi=SL500_SY115&simLd=1
You stick glass pipettes into the bottom of it and roll the wheel with your thumb to draw in liquid. I think the nicotine thins it a lot. I don't have any trouble with using a 16 gauge needle.
I'd replace the Turkey baster with something like this:
https://www.amazon.com/Eisco-Borosilicate-Serological-research-Pipette/dp/B014I2TO0W/ref=mp_s_a_1_6?keywords=25ml+pipette&qid=1572634277&sprefix=25ml+&sr=8-6
And either
https://www.amazon.com/Karter-Scientific-PIPETTE-PUMP-25ml/dp/B00191HNKY/ref=mp_s_a_1_5?keywords=25ml+pipette&qid=1572634277&sprefix=25ml+&sr=8-5
Or
https://www.amazon.com/OESS-Pipette-Filler-Valves-Rubber/dp/B07XNWSFVZ/ref=mp_s_a_1_14?keywords=pipette+with+filler&qid=1572634387&sr=8-14
For pouring I use a stainless steel funnel. But depending on the bottle size, I might also use a 25Ml serological pipette with hand pump.
Downside to the pump & pipette combo, you need steady hands as it's just over 20 inches long, can be a little unwieldy.
My clean environment is simply a glass top table that I wipe down with 10% bleach and 70% ethanol with a little tiki torch burner and I've had great success with minimal contams (2 out of 40 plates). I'm starting LC using a $7 pipettor with a $10 reusable glass serological pipet for transferring between LC and grain jars. I'll also use an inoculating loop to transfer between spore print to agar or agar culture to LC. It's just some methods I'm adopting from things I've learned in microbiology lab courses so I can avoid using disposable syringes.